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KMID : 1041219940360010062
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Journal of Sericultural and Entomological Science
1994 Volume.36 No. 1 p.62 ~ p.68
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Identification and Bioassay of Ice Nucleation Active Bacteria from Branch Rot of Mulberry and Their Populations
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尹馨珠/Yoon, Hyung Joo
金永澤/秦璟植/朴仁均/Kim, Yong Taek/Jin, Kyoung Sik/Park, In Gyun
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Abstract
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Isolation. indentification, bioassay and distribution of ice nucleation active(INA) bacteria were done on branch rot of mulberry which was severely developed after harvest of mulberry branches in autumn. Twelve isolates and two isolates out of thirty-six isolates had ice nucleation aclivity from -5°C to -10°C, and over -5°C, respectively. Isolates which formed ice nucleation from -5°C to -10°C were not inclined to injure tomato and corn seedlings. However, Two isolates, SE9316 and SE9338 which formed ice nucleation over -5°C injured mulberry, tomato and corn seedlings. SE9316 and SE9338 were identified as Pseudomonas syringae based on the mophological, cultural and biochemical characteristics.
Populations of ice nucleation active bacteria, fluorecent pseudomonas, were higer in February and April, but they decreased in May. Populations decreased as they become distant from the center of the symptom. The bacterial populations of all sampling times and sites were higer than 105 cfu/g which was enough to induce frost injury.
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